3T3-L1 小鼠脂肪細胞

簡要描述：3T3-L1 小鼠脂肪細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞。細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件!

產(chǎn)品型號：CL-173
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2024-11-14
訪問量：2566

產(chǎn)品咨詢聯(lián)系我們

產(chǎn)品分類

Product Category

細胞庫

細胞原代牛、狗、雞、鴨、魚原代細胞羊原代細胞豬原代細胞兔原代細胞小鼠原代細胞大鼠原代細胞人原代細胞培養(yǎng)基原代細胞凍存液 ATCC 人乳腺癌豬正常細胞豬睪丸細胞系人膠質(zhì)瘤細胞人成骨細胞人永生化肝細胞人肝癌細胞人卵巢癌細胞人結(jié)直腸腺癌人經(jīng)典小細胞肺癌細胞人小細胞肺癌細胞人結(jié)直腸腺癌細胞人鼻腔上皮細胞中國倉鼠卵巢懸浮細胞嗜性逆轉(zhuǎn)錄病毒包裝穩(wěn)定細胞株小鼠神經(jīng)細胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉(zhuǎn)錄酶病毒包裝細胞鴨子胚胎成纖維細胞豬腎細胞系昆蟲細胞貓腎細胞恒河猴胚腎細胞倉鼠卵巢細胞負鼠腎細胞倉鼠肺細胞果蠅胚胎細胞家貓肺成纖維樣細胞雞胚細胞豚鼠心肌來源細胞豚鼠肺成纖維樣細胞豚鼠皮膚成纖維樣細胞狗腎細胞非洲綠猴胚胎腎上皮細胞猴胚胎腎上皮細胞長臂猿淋巴瘤新生牛眼囊成纖維細胞黑化大家鼠肺成纖維樣細胞俄勒岡地鼠細胞家兔皮膚成纖維樣細胞昆蟲卵巢細胞人胸膜瘤細胞非洲綠猴腎細胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細胞黃胸鼠肺成纖維樣細胞白鰱尾鰭細胞蝙蝠肺細胞草魚腎細胞系大黃魚腎細胞猴腎細胞系犬腎細胞系人結(jié)腸直腸腺癌豬肺泡巨噬細胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導(dǎo)因子人乳腺癌細胞結(jié)腸癌細胞成纖維細胞人胚腎細胞動物卵巢細胞動物胚胎細胞動物肺細胞肋骨來源細胞成纖維樣細胞動物上皮細胞動物肝臟細胞可誘導(dǎo)表達穩(wěn)定細胞株動物氣管細胞動物腎細胞動物巨噬細胞人正常細胞人腫瘤細胞、癌細胞小鼠正常細胞小鼠腫瘤細胞、癌細胞大鼠正常細胞大鼠腫瘤細胞其它動物細胞查看全部產(chǎn)品

相關(guān)文章

詳細介紹

3T3-L1 小鼠脂肪細胞 的詳細介紹

3T3-L1 小鼠脂肪細胞

ATCC^® Number:

CL-173™

Price:

Designations:	3T3-L1
Depositors:	Massachusetts Institute of Technology
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus （mouse）
Morphology:	fibroblast
Source:	Organ: embryo Cell Type: fibroblast
Cellular Products:	triglycerides [3491]
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may CCbe required for the transfer of this ATCC material. Anyone purchasing AT material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host （Nucleofection technology from Lonza Roche FuGENE® Transfection Reagents）
Receptors:	insulin, expressed
Reverse	negative
Age:	embryo
Comments:	L1 is a continuous substrain of 3T3 （Swiss albino） developed through clonal isolation. The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090]. Tested and found negative for ectromelia virus （mousepox）. This line is also designated as ATCC CCL-92.1.ATCC CL-173 was deposited in 1974 without passage number information from the depositor. At the time of submission, ATCC prepared approximay 30 vials of seed stock at about 4 passages beyond the original depositor material （passage number: unknown +4）.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final ation of 10%. Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C Growth Conditions: The serum used is important in culturing this line. Calf serum is recommended and not fetal bovine serum.
Subculturing:	Protocol: Never allow culture to become compley confluent. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. The recommended inoculum is 2 to 3 X 10（3） cells/sq. cm. Subculture before cultures become 70 to 80% confluent or when cells reach 5 to 6 X10（4） viable cells/sq. cm. Incubate cultures at 37C. Interval: Every three days Medium Renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Doubling Time:	14 hrs
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 formerly distributed as:ATCC CCL-92.1 0.25% （w/v） Trypsin - 0.53 mM EDTA in Hank' BSS （w/o Ca++, Mg++）:ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X Recommended serum: ATCC 30-2030
References:	886: Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 4426090 3491: Green H. Triglyceride-accumulating clonal cell line. US Patent 4,003,789 dated Jan 18 1977 32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

產(chǎn)品咨詢

留言框

產(chǎn)品：
您的單位：
您的姓名：
聯(lián)系電話：
常用郵箱：
省份：
詳細地址：
補充說明：
驗證碼：

請輸入計算結(jié)果（填寫阿拉伯?dāng)?shù)字），如：三加四=7

上一篇：CCL-9.1NCTC 1469 小鼠正常肝細胞
下一篇：CCL-226C3H/10T1/2, Clone 8 小鼠胚胎成纖維細胞

91色在线 | 日韩,欧美日本日韩aⅴ在线视频,中文字幕91人妻,丁香花高清在线观看完整版

3T3-L1 小鼠脂肪細胞

留言框

產(chǎn)品：

您的單位：

您的姓名：

聯(lián)系電話：

常用郵箱：

省份：

詳細地址：

補充說明：

驗證碼：